Diagnostic utility of Bcl-2 immunohistochemical expression in pediatric functional bowel obstruction cases with ganglionated specimens

Abstract Background Functional disturbances of the gastrointestinal tract are caused by a number neurodysplastic conditions, including diseases that rarer than Hirschsprung’s disease (HSCR), such as ganglion cell immaturity and intestinal neuronal dysplasia (IND). Bcl-2 shows positive immunoreactivity in degenerative immature cells (IGCs). This work evaluates implication immunohistochemical expression cases pediatric pseudo-obstruction (IPO) with ganglionated specimens. 2-year prospective observational study was conducted from January 2017 to December 2019 on all specimens for referred surgery department clinical data suggesting IPO (abdominal distension > 3 months, intermittent bilious vomiting, no radiological evidence transitional zone HSCR or mechanical obstruction). The exclusion criteria were patients related myopathic disorders, aganglionic biopsies, inadequate tissue blocks. same without used control group. All underwent histologic examination nerve bundles also evaluated expression. Results Twenty-one analyzed, six colonic resection 15 biopsies taken full-thickness transanal biopsy ( n = 12) incisional inadequately functioning stomas 3). mean ± standard deviation age patient cohort 22 7.4 months (range, 19–153 months), there 13 (61.9%) male patients. protein strongly 57.1% cases, weakly 19%, negative 23.8% well 21 A highly significant correlation observed between P < 0.001), where hyperganglionic showed strong positivity compared hypoganglionic adequately ganglionic association found IND other disorders dysganglionosis 0.04) post-HSCR 0.002). Conclusion immunohistochemistry is valuable tool diagnose allied through its IGCs, which difficult identify conventional hematoxylin eosin staining.